Unfolder dlbcl trial3/30/2023 ![]() Interestingly, DSF/Cu significantly reduced BCL6 and AIP levels. Furthermore, we found that the antitumor effect of DSF/Cu against DLBCL cells occurred through ROS-dependent NF-kB signaling pathway, and that the ROS scavenger N-acetyl-l-cysteine (NAC) could reverse the cellular ROS levels. Compared with using DSF alone, the combination of DSF with Cu induced apoptosis in DLBCL cells more dramatically. Meanwhile, DSF or DSF/Cu significantly induced DLBCL cell apoptosis. Results: DSF or DSF/Cu exhibited a marked inhibitory effect on the growth of DLBCL cells, accompanied by cell cycle arrest at the G0/G1 phase. The protein expression were respectively measured by flow cytometry and western blotting. Flow cytometry was performed to evaluate the cell apoptosis levels, cell cycle, the mitochondrial membrane potential and the intracellular accumulation of reactive oxygen species (ROS). ![]() Cell morphology was observed under microscope after Hoechst 33258 and Wright (BaSO) staining. Methods: DLBCL cell lines including OCI-LY1, OCI-LY7, OCI-LY10, and U2932 cell lines and primary DLBCL cells from new-diagnosed DLBCL patients (n=8) were pretreated with DSF alone or in combination with Cu. We here aimed to elucidate the effects and the underlying mechanisms of DSF alone or in combination with copper (Cu) on DLBCL cell lines and primary DLBCL cells. Disulfiram (DSF) has been demonstrated to have antitumor effects on some tumor however, its effect on DLBCL is unclear. Thus, it is imperative to improve the survival of DLBCL patients. Background: The B-cell lymphoma 6 (BCL6) oncogene is required for the survival of diffuse large B-cell lymphoma (DLBCL), which is incurable using conventional chemotherapy.
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